Size  100ul
Cat code  220025
Species Cross-reactivity  H,M,Mk,R
Key Application  WB
Host  Mouse
Clone Number  4E11-F10-H1
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG1
Molecular Weight  19kDa
Sensitivity  The antibody detects endogenous level of total SKP1 protein.
Immunogen  Purified recombinant fragment of human SKP1 expressed in E. Coli.
UniProt Number  P63208
GeneBank ID  NM_006930.3
Subcellular location  
Formulation  Ascitic fluid containing 0.03% sodium azide.
Altername  Cycl Organ of Corti protein II ; OTTHUMP00000159379 ; OTTHUMP00000159380 ; OTTHUMP00000228275 ; OTTHUMP00000228276 ; OTTHUMP00000228278 ; OTTHUMP00000228279 ; OTTHUMP00000228281 ; p19A ; p19skp1 ; RNA polymerase II elongation factor like protein ; RNA polymerase II elongation factor like protein OCP2 ; RNA polymerase II elongation factor-like protein ; S phase kinase associated protein 1 ; S phase kinase associated protein 1A ; S phase kinase associated protein 1A p19A ; S-phase kinase-associated protein 1 ; SIII ; Skp 1 ; SKP 1A ; skp1 ; SKP1_HUMAN ; SKP1A ; TCEB1L ; Transcription Elongation Factor B 1 like ; Transcription elongation factor B ; Transcription elongation factor B SIII polypeptide 1 like ; Cyclin A/CDK2 associated p19 ; Cyclin A/CDK2 associated protein p19 ; Cyclin-A/CDK2-associated protein p19 ; EMC19 ; MGC34403 ; OCP 2 ; OCP II ; OCP II protein ; OCP-2 ; OCP-II ; OCP2 ; OCPII ; Organ of Corti protein 2 ; Organ of Corti protein II ; OTTHUMP00000159379 ; OTTHUMP00000159380 ; OTTHUMP00000228275 ; OTTHUMP00000228276 ; OTTHUMP00000228278 ; OTTHUMP00000228279 ; OTTHUMP00000228281 ; p19A ; p19skp1 ; RNA polymerase II elongation factor like protein ; RNA polymerase II elongation factor like protein OCP2 ; RNA polymerase II elongation factor-like protein ; S phase kinase associated protein 1 ; S phase kinase associated protein 1A ; S phase kinase associated protein 1A p19A ; S-phase kinase-associated protein 1 ; SIII ; Skp 1 ; SKP 1A ; skp1 ; SKP1_HUMAN ; SKP1A ; TCEB1L ; Transcription Elongation Factor B 1 like ; Transcription elongation factor B ; Transcription elongation factor B SIII polypeptide 1 like ;
Gene_symbol  OCP2; p19A; EMC19; SKP1A; OCP-II; TCEB1L
Summary  This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq, Jul 2008]
UniPort summary  Function  Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at ´Lys-21´ and ´Lys-22´; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) direct ubiquitination of TTI1 and TELO2.
Application Images :  
Western blot detection of SKP1 antibody in COS7, Raji, 3T3, PC12 and Hela cell lysates using SKP1 antibody(dilution 1:500).Predicted band size:19KDa.Observed band size:19KDa.