Size  100ug
Cat code  200767-E7
Species Cross-reactivity  H
Key Application  WB,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  25kDa
Sensitivity  This antibody detects endogenous levels of UCHL1 and does not cross-react with related proteins.
Immunogen  Purified recombinant human UCHL1. protein fragments expressed in E.coli.
UniProt Number  P09936
GeneBank ID  NM_004181
Subcellular location  Cytoplasm
Formulation  Purified mouse monoclonal in PBS(pH 7.4) containing  with 0.2% sodium azide, 50% glycerol, 0.1%BSA
Altername  Neuron cytoplasmic protein 9.5；PGP 9.5；Ubiquitin thioesterase L1
Gene_symbol  PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5
Summary  The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.
UniPort summary  Function  Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated
proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of
ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have
ATP-independent ubiquitin ligase activity
Application Images :  
Western blot detection of UCHL1 in U251,Raji&IMP32 cell lysates and  using UCHL1 antibody (1:1000 diluted). Predicted band size: 25KDa Observed band size:25KDa
Immunocytochemistry stain of COS7 using UCHL1 antibody (1:300).