Size  100ug
Cat code  200022
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  43kDa
Sensitivity  This antibody detects endogenous levels of Bmi1 and does not cross-react with related proteins.
Immunogen  Purified recombinant human Bmi1 protein fragments expressed in E.coli.
UniProt Number  P35226
GeneBank ID  NM_005180
Subcellular location  Nucleus. Cytoplasm
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername  B lymphoma Mo MLV insertion region (mouse);B lymphoma Mo MLV insertion region 1 homolog;Bmi 1;BMI1;BMI1 polycomb ring finger oncogene;BMI1_HUMAN;Flvi 2/bmi 1;FLVI2/BMI1;MGC12685;Murine leukemia viral (bmi 1) oncogene homolog;Oncogene BMI 1;PCGF 4;PCGF4;Polycomb complex protein BMI 1;Polycomb complex protein BMI-1;Polycomb group protein Bmi1;Polycomb group ring finger 4;Polycomb group RING finger protein 4;RING finger protein 51;RNF 51;RNF51.
Gene_symbol  PCGF4; RNF51; FLVI2/BMI1
Summary  null
UniPort summary  Function  Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A ´Lys-119´, rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Application Images :  
Western blot detection of Bmi1 antibody in Hela,A549 and 293T cell lysates using Bmi1 antibody (1:1000 diluted).Predicted band size:43KDa.Observed band size:43KDa.