Size  100ug
Cat code  200822
Species Cross-reactivity  H,M
Key Application  WB,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  48kDa
Sensitivity  This antibody detects endogenous levels of TNFRSF10B and does not cross-react with related proteins.
Immunogen  Purified recombinant human TNFRSF10B protein fragments expressed in E.coli.
UniProt Number  O14763
GeneBank ID  NM_003842
Subcellular location  Membrane;Single-pass type I membrane protein.
Formulation  Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername  Fas like protein;Apoptosis inducing protein TRICK2A/2B;Apoptosis inducing receptor TRAIL R2;CD 262;CD262;CD262 antigen;Cytotoxic TRAIL receptor 2;Death domain containing receptor for TRAIL/Apo 2L;Death domain containing receptor for TRAIL/Apo2L;Death receptor 5;DR 5;DR5;Fas like protein precursor;KILLER;KILLER/DR5;OTTHUMP00000123492;OTTHUMP00000123493;p53 regulated DNA damage inducible cell death receptor (killer);p53 regulated DNA damage inducible cell death receptor(killer);TNF related apoptosis inducing ligand receptor 2;TNF related apoptosis inducing ligand receptor 2;TNF-related apoptosis-inducing ligand receptor 2;TNFRSF 10B;TNFRSF10B;TR10B_HUMAN;TRAIL R2;TRAIL receptor 2;TRAIL-R2;TRAILR2;TRANCER;TRICK2;TRICK2A;TRICK2B;TRICKB;Tumor necrosis factor receptor like protein ZTNFR9;Tumor necrosis factor receptor like protein ZTNFR9;Tumor necrosis factor receptor superfamily member 10b;Tumor necrosis factor receptor superfamily, member 10b;ZTNFR9.
Gene_symbol  DR5;CD262;KILLER;TRICK2;TRICKB;ZTNFR9;TRAILR2;TRICK2A;TRICK2B;TRAIL-R2;KILLER/DR5.
Summary  The protein encoded by this gene is a member of the TNF-receptor superfamily,and contains an intracellular death domain.This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand(TNFSF10/TRAIL/APO-2L),and transduces an apoptosis signal.Studies with FADD-deficient mice suggested that FADD,a death domain containing adaptor protein,is required for the apoptosis mediated by this protein.Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene.[provided by RefSeq,Mar 2009]
UniPort summary  Function  Receptor for the cytotoxic ligand TNFSF10/TRAIL.The adapter molecule FADD recruits caspase-8 to the activated receptor.The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.Essential for ER stress-induced apoptosis.
Application Images :  
Western blot detection of TNFRSF10B antibody in Mouse kindey,Mouse brain,HCT116 and Hela cell lysates using TNFRSF10B antibody (1:500-1:2000 diluted).Predicted band size:40/48KDa.Observed band size:40/48KDa.
Immunocytochemistry of HeLa cells fixed by Paraformaldehyde and using anti-TNFRSF10B antibody diluted 1:100.