Size  100ug
Cat code  200270
Species Cross-reactivity  H,M
Key Application  WB,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  43kDa
Sensitivity  This antibody detects endogenous levels Pyruvate Dehydrogenase and does not cross-react with related proteins.
Immunogen  Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.
UniProt Number  P08559
GeneBank ID  NM_000284
Subcellular location  Mitochondrion matrix
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  PDHA; PHE1A; PDHCE1A
Gene_symbol  PDHA; PHE1A; PDHCE1A
Summary  The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniPort summary  Function  The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Application Images :  
Western blot detection of Pyruvate Dehydrogenase(C-terminus) antibody in 293,1299,mEsc&Tc1 cell lysates using Pyruvate Dehydrogenase(C-terminus) antibody (1:1000 diluted).
Predicted band size: 43KDa
Observed band size: 43KDa.
Immunocytochemistry of HeLa cells using anti- Pyruvate Dehydrogenase (C-terminus) antibody diluted 1:150.