Size  100ug
Cat code  230001
Species Cross-reactivity  
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG1
Molecular Weight  kDa
Sensitivity  This antibody specifically recognizes the 6X His-Tag at C-terminal or N-terminal of recombinant proteins,which can be used to detect His-Tag fusion protein of various expression vector.
Immunogen  Synthetic peptide corresponding to fragment (HHHHHH) His Tag
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Summary  The 6xHis (H-H-H-H-H-H) motif is used as a epitope tag on many recombinant proteins for efficient coupling to Ni2  affinity resins and purification by one step chromatography. The antibody recognizes the 6xHis-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Cellular localization: Depends upon the localization of the parent protein tagged with hexahistidine.
UniPort summary  Function  null
Application Images :  
Western blot detection of His-tag antibody in various bacterial lysates,which containing His-tagged proteins (MW: 30 kDa,45 kDa,72 kDa,44 kDa and 40 kDa respectively) using anti-his antibody (1:1000 diluted).