Size  100ul
Cat code  220019
Species Cross-reactivity  H
Key Application  WB,ICC
Host  Mouse
Clone Number  5D7-H1-A8
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG1
Molecular Weight  27kDa
Sensitivity  The antibody detects endogenous level of total hsp27 protein.
Immunogen  Purified recombinant fragment of human HSP27 expressed in E. Coli.
UniProt Number  P04792
GeneBank ID  NM_001540.3
Subcellular location  Cytoplasm. Nucleus. Cytoplasm ? cytoskeleton ? spindle. Note: Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Formulation  Ascitic fluid containing 0.03% sodium azide.
Altername  Heat shock 27kDa protein ; 28 kDa heat shock protein ; CMT2F ; DKFZp586P1322 ; Estrogen regulated 24 kDa protein ; Estrogen-regulated 24 kDa protein ; Heat shock 25kDa protein 1 ; Heat shock 27 kDa protein ; Heat shock 27kD protein 1 ; Heat shock 27kDa protein 1 ; Heat shock 28kDa protein 1 ; Heat Shock Protein 27 ; Heat Shock Protein 27 ; Heat shock protein beta 1 ; Heat shock protein beta-1 ; HMN2B ; HS.76067 ; Hsp 25 ; HSP 27 ; Hsp 28 ; Hsp B1 ; Hsp25 ; HSP27 ; Hsp28 ; HspB1 ; HSPB1_HUMAN ; SRP27 ; Stress responsive protein 27 ; Stress-responsive protein 27 ;
Gene_symbol  CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067
Summary  The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
UniPort summary  Function  Involved in stress resistance and actin organization.
Application Images :  
Western blot detection of HSP27 antibody in Jurkat, HepG2 and Hela cell lysates using HSP27 antibody(dilution 1:500).Predicted band size:27KDa.Observed band size:27KDa.
Immunocytochemistry of HeLa cells using anti-hsp27 antibody diluted 1:100.