Size  100ug
Cat code  200764
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  34kDa
Sensitivity  This antibody detects endogenous levels of CRK and does not cross-react with related proteins.
Immunogen  Purified recombinant human CRK protein fragments expressed in E.coli.
UniProt Number  P46108
GeneBank ID  NM_016823.2
Subcellular location  Cytoplasm, Cell membrane
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  Proto-oncogene c-Crk;
p38;CRKII;adapter molecule crk
Gene_symbol  p38; CRKII 
Summary  This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. 
UniPort summary  Function  The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.
Application Images :  
Western blot detection of CRK in LNCAP,A431，MCF7&HT1080 cell lysates using CRK antibody (1:1000 diluted).
Predicted band size: 34kDa
Observed band size: 34kDa