Size  100ug
Cat code  220005
Species Cross-reactivity  H
Key Application  WB,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG1
Molecular Weight  22kDa
Sensitivity  This antibody detects endogenous levels of BID and does not cross-react with related proteins.
Immunogen  Purified recombinant fragment of human BID expressed in E.Coli.
UniProt Number  P55957
GeneBank ID  NM_001196
Subcellular location  Cytoplasm.Mitochondrion membrane.BH3-interacting domain death agonist p15: Mitochondrion membrane.BH3-interacting domain death agonist p13: Mitochondrion membrane.Isoform 1: Cytoplasm.Isoform 3: Cytoplasm.Isoform 2: Mitochondrion membrane.
Formulation  Ascitic fluid containing 0.03% sodium azide.
Altername  Apoptic death agonist;Apoptotic death agonist BID;BH3 interacting domain death agonist;BH3 interacting domain death agonist p11;BH3 interacting domain death agonist p13;BH3 interacting domain death agonist p15;BH3-interacting domain death agonist p11;BID;BID isoform ES(1b);BID isoform L(2);BID isoform Si6;BID_HUMAN;Desmocollin type 4;FP497;MGC15319;MGC42355;p11 BID;p13 BID;p15 BID;p22 BID.
Gene_symbol  FP497.
Summary  This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2.The encoded protein is a member of the BCL-2 family of cell death regulators.It is a mediator of mitochondrial damage induced by caspase-8 (CASP8);CASP8 cleaves this encoded protein,and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.Multiple alternatively spliced transcript variants have been found,but the full-length nature of some variants has not been defined.[provided by RefSeq,Jul 2008]
UniPort summary  Function  The major proteolytic product p15 BID allows the release of cytochrome c By similarity.Isoform 1,isoform 2 and isoform 4 induce ICE-like proteases and apoptosis.Isoform 3 does not induce apoptosis.Counters the protective effect of Bcl-2.
Application Images :  
Western blot detection of BID antibody in 293T,Jurkat and A431 cell lysates using BID antibody (1:300 diluted).Predicted band size:22KDa.Observed band size:22KDa.
Immunocytochemistry staining of HeLa cells using anti-BID antibody (dilution 1:100).