Size  100ug
Cat code  200722
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  70kDa
Sensitivity  This antibody detects endogenous levels of BIRC2 and does not cross-react with related proteins.
Immunogen  Purified recombinant human BIRC2 protein fragments expressed in E.coli.
UniProt Number  Q13490
GeneBank ID  NM_001166.3
Subcellular location  Cytoplasm. Nucleus.
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
Altername  C-IAP1；IAP homolog B；Inhibitor of apoptosis protein 2，Short name=IAP-2，Short name=hIAP-2，Short name=hIAP2；RING finger protein 48；TNFR2-TRAF-signaling complex protein 2;BIRC2;
Gene_symbol  API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
Summary  The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniPort summary  Function  Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO and MXD1/MAD1. Can also function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle.
Application Images :  
Western blot detection of BIRC2 in K562 cell lysates and  using BIRC2 antibody (1:1000 diluted). 
Predicted band size: 70KDa 
Observed band size: 70KDa