Size  100ul
Cat code  220032
Species Cross-reactivity  H, R, M
Key Application  WB, ICC, IP
Host  Mouse
Clone Number  4E7-E8-C2
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG2b
Molecular Weight  67kDa
Sensitivity  The antibody detects endogenous level of AIFM1 protein.
Immunogen  Purified recombinant fragment of human AIF expressed in E. Coli.
UniProt Number  O95831
GeneBank ID  NM_001130846
Subcellular location  Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm ? perinuclear region. Note: Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.Isoform 5: Cytoplasm.
Formulation  Ascitic fluid containing 0.03% sodium azide.
Altername  AIFM1; AIFM1_HUMAN; Apoptosis inducing factor 1, mitochondrial; Apoptosis inducing factor; Apoptosis inducing factor, mitochondrion associated, 1; Apoptosis-inducing factor 1; CMTX4; COXPD6; Harlequin; Hq; mAIF; MGC111425; MGC5706; mitochondrial; PDCD 8; PDCD8; Programmed cell death 8 (apoptosis inducing factor); Programmed cell death 8; Programmed cell death 8 isoform 1; Programmed cell death 8 isoform 2; Programmed cell death 8 isoform 3; Programmed cell death protein 8; Programmed cell death protein 8 mitochondrial; Programmed cell death protein 8 mitochondrial precursor; Striatal apoptosis inducing factor;
Gene_symbol  AIF; CMTX4; COWCK; PDCD8; COXPD6
Summary  This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, May 2010]
UniPort summary  Function  Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces ´parthanatos´ i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Application Images :  
Western blot detection of AIF antibody in C6, COS7,3T3 and Hela cell lysates using AIF antibody (dilution 1:1000).Predicted band size:67 KDa.Observed band size:67KDa.
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-AIFM1 antibody (dilution 1:100).
Immunoprecipitation analysis of Hela cell lysates using AIF antibody.