Size  100ug
Cat code  200138
Species Cross-reactivity  H,M
Key Application  WB,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  26kDa
Sensitivity  This antibody detects endogenous levels of CBX5 and does not cross-react with related proteins
Immunogen  Purified recombinant human CBX5 protein fragments expressed in E.coli.
UniProt Number  P45973
GeneBank ID  NM_012117
Subcellular location  Nucleus. Chromosome
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  HP1; HP1A;CBX5
Gene_symbol  HP1; HP1A
Summary  This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.
UniPort summary  Function  Component of heterochromatin that recognizes and binds histone H3 tails methylated at ´Lys-9´ (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when ´Tyr-41´ of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins
Application Images :  
Western blot detection of CBX5 in MCF7,293T,HelaNE,3T3，C6，COS7&Mouse brain  lysates and  using CBX5 antibody (1:1000 diluted). 
Predicted band size: 22KDa 
Observed band size: 22KDa
Immunocytochemistry stain of Hela using CBX5 antibody (1:300).