Size  100ul
Cat code  220028
Species Cross-reactivity  H
Key Application  WB, ICC
Host  Mouse
Clone Number  8E2-F3-D2
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG1
Molecular Weight  82kDa
Sensitivity  The antibody detects endogenous level of CD44 protein.
Immunogen  Purified recombinant fragment of human CD44 (628-699) expressed in E. Coli.
UniProt Number  P16070
GeneBank ID  NM_001001389
Subcellular location  Membrane; Single-pass type I membrane protein. Note: Colocalizes with actin in membrane protrusions at wounding edges
Formulation  Ascitic fluid containing 0.03% sodium azide.
Altername  LHR. LHR; LHR; BA-1; CD 44; CD44; CD44; CD44 antigen; CD44 molecule (Indian blood group); CD44 molecule; CD44_HUMAN; CDw44; Cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HSA; HUTCH-I; HUTCH1; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU3; MGC10468; MIC4; MUTCH1; PGP-1; PGP-I; PGP1; Phagocytic glycoprotein 1; Phagocytic glycoprotein I;
Gene_symbol  N; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Summary  The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]
UniPort summary  Function  Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Application Images :  
Western blot detection of CD44 antibody in COS7, HUVEC and Hela cell lysates using CD44 antibody (dilution 1:1000).Predicted band size:82 KDa.Observed band size:82KDa.
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-CD44 antibody (dilution 1:100).