Size  100ul
Cat code  200838
Species Cross-reactivity  H,M,R,Mk
Key Application  WB,ICC
Host  Mouse
Clone Number  1A7-E11-E9
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2a
Molecular Weight  38kDa
Sensitivity  This antibody detects endogenous levels of PRKAB1 and does not cross-react with related proteins.
Immunogen  Purified recombinant human PRKAB1 protein fragments expressed in E.coli.
UniProt Number  Q9Y478
GeneBank ID  NM_006253.4
Subcellular location  
Formulation  Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Altername  1300015D22Rik;5 AMP activated protein kinase subunit beta 1;5´´-AMP-activated protein kinase subunit beta-1;AAKB1_HUMAN;AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1;AMP-activated, noncatalytic, beta-1;AMPK;AMPK beta 1 chain;AMPK subunit beta-1;AMPK-BETA-1;AMPKb;AU021155;E430008F22;HAMPKb;MGC17785;PRKAB1.
Gene_symbol  AMPK;HAMPKb.
Summary  The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK).AMPK is a heterotrimer consisting of an alpha catalytic subunit,and non-catalytic beta and gamma subunits.AMPK is an important energy-sensing enzyme that monitors cellular energy status.In response to cellular metabolic stresses,AMPK is activated,and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR),key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol.This subunit may be a positive regulator of AMPK activity.The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.[provided by RefSeq,Jul 2008]
UniPort summary  Function  Non-catalytic subunit of AMP-activated protein kinase (AMPK),an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism.In response to reduction of intracellular ATP levels,AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein,carbohydrate and lipid biosynthesis,as well as cell growth and proliferation.AMPK acts via direct phosphorylation of metabolic enzymes,and by longer-term effects via phosphorylation of transcription regulators.Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton;probably by indirectly activating myosin.Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles,via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1,PRKAG2 or PRKAG3).
Application Images :  
Western blot detection of PRKAB1 antibody in 3T3,Hela,PC-12,COS7 and MDA-MB-468 cell lysates using PRKAB1 antibody (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min.
Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using anti-PRKAB1 antibody (dilution 1:100).