Size  100ug
Cat code  310002
Species Cross-reactivity  M,Mk,R
Key Application  WB
Host  Rabbit
Antibody type  Polyclonal antibody
Purified method  Affinity purified
Isotype  
Molecular Weight  91kDa
Sensitivity  The antibody detects endogenous level of total STAT1 protein.
Immunogen  Peptide sequence around aa. 699~703 (T-G-Y-I-K) derived from Human STAT1.
UniProt Number  P42224
GeneBank ID  NM_007315.3
Subcellular location  Cytoplasm. Nucleus. Note: Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to IFN-gamma and signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4.
Formulation  Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Altername  CANDF7 ;DKFZp686B04100 ;ISGF 3 ;
Gene_symbol  CANDF7; ISGF-3; STAT91
Summary  The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
UniPort summary  Function  Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
Application Images :  
Western blot detection of STAT1 antibody in C6, COS7, 3T3UV cell lysates using MEK-2 antibody(dilution 1:300). Predicted band size: 91KDa. Observed band size: 91KDa.