Size  100ul
Cat code  220012
Species Cross-reactivity  H,Mk
Key Application  WB
Host  Mouse
Clone Number  4F2-E8-C3
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG1
Molecular Weight  54kDa
Sensitivity  The antibody detects endogenous level of total vimentin protein.
Immunogen  Purified recombinant fragment of Vimentin (aa2-466) expressed in E. Coli.
UniProt Number  P08670
GeneBank ID  NM_003380
Subcellular location  Cytoplasm
Formulation  Ascitic fluid containing 0.03% sodium azide.
Altername  FLJ36605 ; VIM ; VIME_HUMAN ; vimentin ;
Gene_symbol  CTRCT30
Summary  This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
UniPort summary  Function  Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. 
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Application Images :  
Western blot detection of VIM antibody in U2OS, 293, COS7 and Hela cell lysates using VIM antibody(dilution 1:500).Predicted band size:54KDa.Observed band size:54KDa.