Size  100ug
Cat code  200228
Species Cross-reactivity  H
Key Application  WB,IHC,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2a
Molecular Weight  34kDa
Sensitivity  This antibody detects endogenous levels Protein Phosphatase 4C and does not cross-react with related proteins.
Immunogen  Purified recombinant human Protein Phosphatase 4C protein fragments expressed in E.coli.
UniProt Number  P60510
GeneBank ID  NM_002720
Subcellular location  Cytoplasm. Nucleus
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  PP4; PPX; PP4C; PPH3; PPP4;Protein Phosphatase 4C
Gene_symbol  PP4; PPX; PP4C; PPH3; PPP4
Summary  Protein phosphatase 4C may be involved in microtubule organization. It binds 1 iron ion and 1 manganese ion per subunit. PP4 consists of a catalytic subunit PPP4C and a regulatory subunit. PPP4R1 and belongs to the PPP phosphatase family, PP X subfamily.
UniPort summary  Function  Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity)
Application Images :  
Western blot detection of Protein Phosphatase 4C antibody in Hela,293&Jurkat cell lysates using Protein Phosphatase 4C antibody (1:1000 diluted).
Predicted band size: 34KDa
Observed band size: 34KDa
Immunocytochemistry of HeLa cells using anti- Protein Phosphatase 4C antibody diluted 1:200.
IHC of paraffin-embedded human breast cancer using anti-Protein Phosphatase 4C diluted 1/500-1/1000.