Size  100ug
Cat code  200943
Species Cross-reactivity  EGFP,EYFP
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  According to the fused proteinkDa
Sensitivity  Transfected
Immunogen  Purified recombinant EYFP.tag full length expressed in E.coli.
UniProt Number  C8CHS1
GeneBank ID  AAB08060.1
Subcellular location  null
Formulation  Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Altername  GFP;Green Fluorescent Protein;enhanced Green Fluorescent Protein
Gene_symbol  null
Summary  The green fluorescent protein (GFP) is a protein isolated from the jellyfish Aequorea victoria composed of 238 amino acid residues that exhibits bright green fluorescence. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations. Due to the potential for widespread usage and the evolving needs of researchers, many different mutants of GFP have been engineered. For example, EGFP contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr which result in  brighter green fluorescence. EYFP contains the four amino acid substitutions of Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. EYFP emits yellow fluorescence excited by green light.
UniPort summary  Function  null
Application Images :  
Western blot detection of EYFP expression in Rosetta E.coli cells induced by 0(A)or 0.1mM (B)IPTG using EGFP antibody(1:1000 diluted).Predicted band size: 30KDa Observed band size: 30KDa.
Western blot detection of EGFP expression in Hela cells non-transfected (A) or transfected (B) with pEGFP C1 using EGFP antibody(1:5000 diluted).Predicted band size: 34KDa Observed band size: 34KDa.
Western blot detection of EGFP expression in 293T cells transfected (A:mRFP) or transfected (B:EGFP fused protein;C:EGFP) using EGFP antibody(L:1/60000 diluted. M:1/20000 diluted. R:1/10000 diluted).