Size  100ug
Cat code  200359
Species Cross-reactivity  H
Key Application  WB,ICC,FC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2a
Molecular Weight  120kDa
Sensitivity  This antibody detects endogenous levels ATP-Citrate Lyase and does not cross-react with related proteins.
Immunogen  Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli.
UniProt Number  P53396
GeneBank ID  NM_001096
Subcellular location  Cytoplasm
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  ACL; ATPCL; CLATP
Gene_symbol  ACL; ATPCL; CLATP
Summary  ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.
UniPort summary  Function  ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
Application Images :  
Western blot detection of ATP-Citrate Lyase antibody in K562&Hela cell lysates using ATP-Citrate Lyase antibody (1:1000 diluted).
Predicted band size: 120KDa
Observed band size: 120KDa
Immunocytochemistry of HeLa cells using anti- ATP-Citrate Lyase (C-terminus) antibody diluted 1:150.
Flow Cytometry analysis of HeLa cells stained with ATP-Citrate Lyase  (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG