Size  100ug
Cat code  200639
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  42kDa
Sensitivity  This antibody detects endogenous levels of PRMT1 and does not cross-react with related proteins.
Immunogen  Purified recombinant human PRMT1 protein fragments expressed in E.coli.
UniProt Number  Q99873
GeneBank ID  NM_001536.2 
Subcellular location  Nucleus. Cytoplasm 
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  Histone-arginine N-methyltransferase PRMT1
EC=2.1.1.125
Interferon receptor 1-bound protein 4
Gene_symbol  ANM1; HCP1; IR1B4; HRMT1L2 
Summary  This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5
UniPort summary  Function  Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 ´Arg-4´ (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.
Application Images :  
Western blot detection of PRMT1 in Hela&A549 cell lysates using PRMT1 antibody (1:1000 diluted).
Predicted band size:42KDa
Observed band size:42KDa.