Size  100ug
Cat code  200560
Species Cross-reactivity  H,R,M,Mk
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  null
Isotype  IgG1
Molecular Weight  25kDa
Sensitivity  This antibody detects endogenous levels of GRB2 and does not cross-react with related proteins.
Immunogen  Purified recombinant human GRB2 protein fragments expressed in E.coli.
UniProt Number  P62993
GeneBank ID  NM_002086
Subcellular location  Nucleus. Cytoplasm. Endosome. Golgi apparatus By similarity
Formulation  Ascites
Altername  Abundant SRC homology;Adapter protein GRB2;ASH;Ash protein;EGFRBP GRB2;Epidermal growth factor receptor binding protein;Epidermal growth factor receptor binding protein GRB2;GRB 2;GRB2 adapter protein;Grb2;GRB2_HUMAN;Grb3 3;Growth Factor Receptor Bound Protein 2;Growth Factor Receptor Bound Protein 2;Growth factor receptor bound protein 3;Growth factor receptor-bound protein 2;HT027;MST084;MSTP084;OTTHUMP00000166096;OTTHUMP00000166097;OTTHUMP00000166098;Protein ASH;SEM5;SEM5;SH2/SH3 adapter GRB2;
Gene_symbol  ASH;Grb3-3;MST084;NCKAP2;MSTP084;EGFRBP-GRB2
Summary  The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniPort summary  Function  Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. Ref.1 Ref.3 Ref.54

Isoform 2 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform 2 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
Application Images :  
Western blot detection of GRB2 in Jurkat,Raji,PC-12&3T3 cell lysates and using GRB2 antibody(1:500 diluted).Predicted band size: 25KDa Observed band size: 25KDa
Western blot detection of GRB2 in MCF7,3T3,COS7,C6 and Hela cell lysates and using GRB2 antibody(1:300 diluted).Predicted band size: 25KDa.Observed band size: 25KDa.