Size  100ug
Cat code  200357
Species Cross-reactivity  M
Key Application  WB,ICC,FC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  52kDa
Sensitivity  This antibody detects endogenous levels SMAD5 and does not cross-react with related proteins.
Immunogen  Purified recombinant human SMAD5 protein fragments expressed in E.coli.
UniProt Number  Q99717
GeneBank ID  NM_001001420
Subcellular location  Cytoplasm. Nucleus
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  DWFC; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323
Gene_symbol  DWFC; JV5-1; MADH5
Summary  SMAD5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. It is a receptor-regulated SMAD (R-SMAD), and acts as an intracellular signal transducer for the transforming growth factor beta superfamily. SMAD5 is activated through serine phosphorylation by BMP (bone morphogenetic proteins) type 1 receptor kinase. It is cytoplasmic in the absence of its ligand and migrates into the nucleus upon phosphorylation and complex formation with SMAD4. Here the SMAD5/SMAD4 complex stimulates the transcription of target genes.
UniPort summary  Function  Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Application Images :  
Western blot detection of SMAD5 antibody in Mouse testis cell lysates using SMAD5 antibody (1:1000 diluted).
Predicted band size:55KDa
Observed band size:55KDa
Immunocytochemistry of HeLa cells using anti- SMAD5 (C-terminus) antibody diluted 1:75.
Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.