Size  100ug
Cat code  200532
Species Cross-reactivity  H,M
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  95kDa
Sensitivity  This antibody detects endogenous levels of BRAF  and does not cross-react with related proteins.
Immunogen  Purified recombinant human BRAF  protein fragments expressed in E.coli.
UniProt Number  P15056
GeneBank ID  NM_004333
Subcellular location  Nucleus.Cytoplasm.Cell membrane.
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername  FLJ95109;94 kDa B raf protein;B raf 1;B Raf proto oncogene serine threonine protein kinase;BRAF 1;BRAF;BRAF_HUMAN;BRAF1;cRmil;FLJ95109;MGC126806;MGC138284;Murine sarcoma viral (v-raf) oncogene homolog B1;Murine sarcoma viral v raf oncogene homolog B1;NS7;p94;Proto-oncogene B-Raf;Proto-oncogene c-Rmil;RAFB 1;RAFB1;Serine/threonine-protein kinase B-raf;v raf murine sarcoma viral oncogene homolog B1;v-Raf murine sarcoma viral oncogene homolog B1.
Gene_symbol  NS7; BRAF1; RAFB1; B-RAF1
Summary  This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
UniPort summary  Function  Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
Application Images :  
Western blot detection of BRAF in Hela,Ramos,K562,C2C12 & Jurkat cell lysates using BRAF antibody (1:300 diluted).Predicted band size: 86KDa.Observed band size: 95KDa.