Size  100ul
Cat code  220011
Species Cross-reactivity  H,R,M,Mk
Key Application  WB,ICC
Host  Mouse
Clone Number  7G6-C5-D8
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG2a
Molecular Weight  48.8kDa
Sensitivity  The antibody detects endogenous level of total HDAC3 protein. The antibody detects endogenous level of total HDAC3 protein.
Immunogen  Purified recombinant fragment of human HDAC3 (aa224-428) expressed in E. Coli.
UniProt Number  O15379
GeneBank ID  NM_003883
Subcellular location  Nucleus.
Formulation  Ascitic fluid containing 0.03% sodium azide.
Altername  HD 3 ; HD3 ; HDAC 3 ; HDAC3 ; HDAC3_HUMAN ; Histone deacetylase 3 (HD3) (RPD3-2) ; histone deacetylase 3 ; RPD 3 ; RPD3 2 ; RPD3 ; RPD3-2 ; SMAP 45 ; SMAP45 ;
Gene_symbol  HD3; RPD3; RPD3-2
Summary  Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]
UniPort summary  Function  Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation
Application Images :  
Western blot detection of HDAC3 antibody in COS7, C6, Hela and 3T3 cell lysates using HDAC3 antibody(dilution 1:500).Predicted band size:48.8KDa.Observed band size:48.8KDa.
Immunocytochemistry of HeLa cells using anti-HDAC3 antibody diluted 1:100.