Size  100ul
Cat code  220017
Species Cross-reactivity  H,R
Key Application  WB
Host  Mouse
Clone Number  7H12-E4-F5
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG1
Molecular Weight  135kDa
Sensitivity  The antibody detects endogenous level of total CDH1 protein.
Immunogen  Purified recombinant fragment of human CDH1 expressed in E. Coli.
UniProt Number  P12830
GeneBank ID  NM_004360.3
Subcellular location  Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus ? trans-Golgi network. Note: Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
Formulation  Ascitic fluid containing 0.03% sodium azide.
Altername  Arc 1 ; CADH1_HUMAN ; Cadherin 1 ; cadherin 1 type 1 E-cadherin ; Cadherin1 ; CAM 120/80 ; CD 234 ; CD 324 ; CD324 ; CD324 antigen ; CDH1 ; CDHE ; E-Cad/CTF3 ; E-cadherin ; ECAD ; Epithelial cadherin ; epithelial calcium dependant adhesion protein ; LCAM ; Liver cell adhesion molecule ; UVO ; Uvomorulin ;
Gene_symbol  UVO; CDHE; ECAD; LCAM; Arc-1; CD324
Summary  This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008]
UniPort summary  Function  Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. 
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
Application Images :  
Western blot detection of CDH1 antibody in C6 and HaCat cell lysates using CDH1 antibody(dilution 1:500).Predicted band size:135KDa.Observed band size:135KDa.