Size  100ug
Cat code  200541
Species Cross-reactivity  H
Key Application  WB,FC,ICC,IP
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  69kDa
Sensitivity  This antibody detects endogenous levels of TORC1(N-terminus) and does not cross-react with related proteins.
Immunogen  Purified recombinant human TORC1(N-terminus) protein fragments expressed in E.coli.
UniProt Number  Q6UUV9
GeneBank ID  NM_015321
Subcellular location  Cytoplasm. Nucleus
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  KIAA0616;CREB regulated transcription coactivator 1;CREB-regulated transcription coactivator 1;CRTC1;CRTC1_HUMAN;FLJ14027;KIAA0616;MECT 1;Mucoepidermoid carcinoma translocated 1;Mucoepidermoid carcinoma translocated protein 1;TORC-1;TORC1;Transducer of CREB protein 1;Transducer of regulated cAMP response element binding protein 1;Transducer of regulated cAMP response element-binding protein (CREB) 1;Transducer of regulated cAMP response element-binding protein 1;WAMTP1;
Gene_symbol  MECT1; TORC1; WAMTP1
Summary  null
UniPort summary  Function  Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 ´Ser-133´ phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses (By similarity)
Application Images :  
Western blot detection of TORC1(N-terminus) antibody in Hela&Jurkat lysates using TORC1(N-terminus) antibody (1:1000 diluted).
Predicted band size: 69KDa
Observed band size: 69KDa.
Immunocytochemistry stain of Hela using TORC1  antibody (1:300).
Immunoprecipitation analysis of Hela cell lysate using CRTC1 antibody.
Flow Cytometry analysis of K562 cells stained with TORC1(N-terminus) (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.