Size  100ug
Cat code  200171
Species Cross-reactivity  H
Key Application  WB,IHC,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  42kDa
Sensitivity  This antibody detects endogenous levels PRMT6 and does not cross-react with related proteins.
Immunogen  Purified recombinant human PRMT6 protein fragments expressed in E.coli.
UniProt Number  Q96LA8
GeneBank ID  NM_018137
Subcellular location  Nucleus
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  HRMT1L6; FLJ10559; FLJ51477
Gene_symbol  HRMT1L6
Summary  PRMT6 is a protein arginine N-methyltransferase, and catalyzes the sequential transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation is a prevalent post-translational modification in eukaryotic cells that has been implicated in signal transduction, the metabolism of nascent pre-RNA, and the transcriptional activation processes.
IPRMT6 is functionally distinct from two previously characterized type I enzymes, PRMT1 and PRMT4. In addition, PRMT6 displays automethylation activity; it is the first PRMT to do so. PRMT6 has been shown to act as a restriction factor for HIV replication.
UniPort summary  Function  Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 ´Arg-2´ to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 ´Lys-4´ (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 ´Arg-3´ (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC).
Application Images :  
Western blot detection of PRMT6 in Hela&293T cell lysates using PRMT6 antibody (1:1000 diluted).
Predicted band size: 42KDa
Observed band size: 42KDa
Immunocytochemistry stain of Hela using PRMT6 antibody (1:300).
Immunohistochemistry stain of paraffin-embedded human breast cancer using PRMT6 antibody (1:200).