Size  100ug
Cat code  200789
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2a
Molecular Weight  72kDa
Sensitivity  This antibody detects endogenous levels of NRF1,and does not cross-react with related proteins.
Immunogen  Purified recombinant NRF1 protein fragments expressed in E.coli
UniProt Number  Q16656
GeneBank ID  NM_001040110
Subcellular location  Nucleus.
Formulation  Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol,0.1%BSA.
Altername  alpha pal;alpha palindromic binding protein;Alpha-pal;locus control region factor 1;NFE2 related factor 1;NRF-1;Nrf1;nuclear factor (erythroid derived 2)-like 1;nuclear respiratory factor 1;transcription factor 11;
Gene_symbol  ALPHA-PAL
Summary  This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration,heme biosynthesis,and mitochondrial DNA transcription and replication.The protein has also been associated with the regulation of neurite outgrowth.Alternate transcriptional splice variants,which encode the same protein, have been characterized.Additional variants encoding different protein isoforms have been described but they have not been fully characterized.Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for ""nuclear factor(erythroid-derived 2)-like 1"" which has an official symbol of NFE2L1.[provided by RefSeq, Jul 2008]"
UniPort summary  Function  Transcription factor that activates the expression of the EIF2S1(EIF2-alpha)gene.Links the transcriptional modulation of key metabolic genes to cellular growth and development.Implicated in the control of nuclear genes required for respiration,heme biosynthesis,and mitochondrial DNA transcription and replication.
Application Images :  
Western blot detection of NRF1 in Hela,K562,CEM&U2OS cell lysates using NRF1 antibody(1:1000 diluted).Predicted band size:67KDa,Observed band size:72KDa