Size  100ug
Cat code  310004
Species Cross-reactivity  H,Mk
Key Application  WB
Host  Rabbit
Antibody type  Polyclonal antibody
Purified method  Affinity purified
Isotype  
Molecular Weight  26kDa
Sensitivity  The antibody detects endogenous level of total BCL-2 protein.
Immunogen  Peptide sequence around aa. 54~58 (G-H-T-P-H) derived from Human BCL-2.
UniProt Number  P10415
GeneBank ID  NM_000633.2
Subcellular location  Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane;
Formulation  Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Altername  Apoptosis regulator Bcl 2 ;Apoptosis regulator Bcl-2 ;Apoptosis regulator Bcl2 ; AW986256 ;B cell CLL/lymphoma 2 ;B cell leukemia/lymphoma 2 ;B cell lymphoma 2 ;Bcl 2 ;Bcl-2 ;Bcl2 ;BCL2 protein ;BCL2_HUMAN ;C430015F12Rik ;D630044D05Rik ;D830018M01Rik ;Leukemia/lymphoma, B-cell, 2 ;Oncogene B-cell leukemia 2 ;PPP1R50 ;Protein phosphatase 1, regulatory subunit 50 .
Gene_symbol  Bcl-2; PPP1R50
Summary  This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]
UniPort summary  Function  Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Application Images :  
Western blot detection of BCL2 antibody in a549, Hela and COS7 cell lysates using BCL2 antibody(dilution 1:300).Predicted band size:26 Kda.Observed band size:26KDa.