Size  100ul
Cat code  200282
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Clone Number  4E11-E11-G11
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  220kDa
Sensitivity  This antibody detects endogenous levels of SMARCA4 and does not cross-react with related proteins.
Immunogen  Purified recombinant human SMARCA4 protein fragments expressed in E.coli.
UniProt Number  P51532
GeneBank ID  NM_001128844
Subcellular location  Nucleus
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername  ATP dependent helicase SMARCA4;ATP-dependent helicase SMARCA4;BAF 190;BAF190;BAF190A;Brahma protein homolog 1;Brahma protein like 1;BRG1;BRG1 associated factor 190A;BRG1 protein;BRG1-associated factor 190A;BRM/SWI2 related gene 1;Global transcription activator homologous sequence;global transcription activator snf2l4;Homeotic gene regulator;hSNF2b;Mitotic growth and transcription activator;MRD16;Nuclear protein GRB1;Protein brahma homolog 1;Protein BRG-1;Protein BRG1;RTPS2;SMARC A4;SMARCA4;SMCA4_HUMAN;SNF2;SNF2 beta;SNF2 like 4;SNF2-beta;SNF2B;SNF2L4;SNF2LB;Sucrose nonfermenting like 4;SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4;SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a,member 4;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4;SWI2;Transcription activator BRG1.
Gene_symbol  BRG1;SNF2;SWI2;MRD16;RTPS2;BAF190;SNF2L4;SNF2LB;hSNF2b;BAF190A.
Summary  The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes.The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI,which is required for transcriptional activation of genes normally repressed by chromatin.In addition, this protein can bind BRCA1,as well as regulate the expression of the tumorigenic protein CD44.Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2.Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq,May 2012]
UniPort summary  Function  Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation.Component of the CREST-BRG1 complex,a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex.In resting neurons,transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex.Upon calcium influx,RB1 is dephosphorylated by calcineurin,which leads to release of the repressor complex.At the same time,there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism,which leads to transcriptional activation.The CREST-BRG1 complex also binds to the NR2B promoter,and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP.Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex).During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF).The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells.The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals,while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues By similarity.Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex,a chromatin-remodeling complex recruited by vitamin D receptor (VDR),which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.
Application Images :  
Western blot detection of SMARCA4 antibody in Hela NE and K562 cell lysates using SMARCA4 antibody (1:1000 diluted).Predicted band size:220KDa.Observed band size:220KDa.