Size  100ug
Cat code  200090
Species Cross-reactivity  H
Key Application  WB,IP
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  150kDa
Sensitivity  This antibody detects endogenous levels of Dynactin 1(N-terminus),and does not cross-react with related proteins.
Immunogen  Purified recombinant Dynactin 1 protein fragments expressed in E.coli
UniProt Number  Q14203
GeneBank ID  NM_004082
Subcellular location  Cytoplasm
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150mM NaCl)with 0.2% sodium azide,0.1%BSA,50% glycerol.
Altername  150 kDa dynein-associated polypeptide DAP-150;Dynactin 1;DCTN1
Gene_symbol  P135; DP-150; DAP-150
Summary  This gene encodes the largest subunit of dynactin,a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150kD.Dynactin binds to both microtubules and cytoplasmic dynein.Dynactin is involved in a diverse array of cellular functions,including ER-to-Golgi transport,the centripetal movement of lysosomes and endosomes,spindle formation,chromosome movement,nuclear positioning, and axonogenesis.This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus.Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).[provided by RefSeq, Oct 2008]
UniPort summary  Function  Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.
Application Images :  
Western blot detection of DCTN1 in A2780,TF-1,HepG2,MCF7,A431&K562 cell lysate(1:1000 diluted).
Predicted band size:150KDa
Observed band size: 150KDa
Immunoprecipitation analysis of Hela cell lysates using DCTN1 antibody