Size  100ug
Cat code  200551
Species Cross-reactivity  H,M
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  80kDa
Sensitivity  This antibody detects endogenous levels of ADRBK1(C-term.) and does not cross-react with related proteins.
Immunogen  Purified recombinant human ADRBK1(C-term.) protein fragments expressed in E.coli.
UniProt Number  P25098
GeneBank ID  NM_001619
Subcellular location  null
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  beta-ARK-1
G-protein coupled receptor kinase 2
Gene_symbol  GRK2; BARK1; BETA-ARK1
Summary  The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]
UniPort summary  Function  Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
Application Images :  
Western blot detection of ADRBK1  in U937,Raw264.7&Ramos whole cell lysate(1:1000 diluted)
Predicted band size:80KDa.
Observed band size:80KDa.