Size  100ug
Cat code  220003
Species Cross-reactivity  H,M
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG2b
Molecular Weight  42/44kDa
Sensitivity  This antibody detects endogenous levels of p42/44MAPK and does not cross-react with related proteins.
Immunogen  Purified recombinant fragment of human MAPK1 expressed in E. Coli.
UniProt Number  P28482
GeneBank ID  NM_002746
Subcellular location  Cytoplasm ? cytoskeleton ? spindle.Nucleus.Cytoplasm ? cytoskeleton ? centrosome.Cytoplasm.
Formulation  Ascitic fluid containing 0.03% sodium azide.
Altername  ERK1;p38;p40;p41;ERK2;ERT1;MAPK2;PRKM1;PRKM2;P42MAPK;p41mapk;MAPK1.
Gene_symbol  ERK;p38;p40;p41;ERK2;ERT1;MAPK2;PRKM1;PRKM2;P42MAPK;p41mapk;MAPK1.
Summary  The protein encoded by this gene is a member of the MAP kinase family.MAP kinases,also known as extracellular signal-regulated kinases (ERKs),act as an integration point for multiple biochemical signals,and are involved in a wide variety of cellular processes such as proliferation,differentiation,transcription regulation and development.The activation of this kinase requires its phosphorylation by upstream kinases.Upon activation,this kinase translocates to the nucleus of the stimulated cells,where it phosphorylates nuclear targets.Two alternatively spliced transcript variants encoding the same protein,but differing in the UTRs,have been reported for this gene.[provided by RefSeq,Jul 2008]
UniPort summary  Function  Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway.MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade.They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF.Depending on the cellular context,the MAPK/ERK cascade mediates diverse biological functions such as cell growth,adhesion,survival and differentiation through the regulation of transcription,translation,cytoskeletal rearrangements.The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis,mitosis,and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors.About 160 substrates have already been discovered for ERKs.Many of these substrates are localized in the nucleus,and seem to participate in the regulation of transcription upon stimulation.However,other substrates are found in the cytosol as well as in other cellular organelles,and those are responsible for processes such as translation,mitosis and apoptosis.Moreover,the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics,including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC);as well as in the fragmentation of the Golgi apparatus during mitosis.The substrates include transcription factors (such as ATF2,BCL6,ELK1,ERF,FOS,HSF4 or SPZ1),cytoskeletal elements (such as CANX,CTTN,GJA1,MAP2,MAPT,PXN,SORBS3 or STMN1),regulators of apoptosis (such as BAD,BTG2,CASP9,DAPK1,IER3,MCL1 or PPARG),regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2,DCC,FRS2 or GRB10) Protein kinases (such as RAF1,RPS6KA1/RSK1,RPS6KA3/RSK2,RPS6KA2/RSK3,RPS6KA6/RSK4,SYK,MKNK1/MNK1,MKNK2/MNK2,RPS6KA5/MSK1,RPS6KA4/MSK2,MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1,DUSP4,DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets,thereby extending the specificity of the cascade.May play a role in the spindle assembly checkpoint.
Application Images :  
Western blot detection of p42/44MAPK antibody in Hela,3T3 and Raw264.7 cell lysates using p42/44MAPK antibody (1:300 diluted).Predicted band size:42/44KDa.Observed band size:42/44KDa.