Size  100ug
Cat code  200083
Species Cross-reactivity  H
Key Application  WB,IHC,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  37kDa
Sensitivity  This antibody detects endogenous levels of TFIIB and does not cross-react with related proteins
Immunogen  Purified recombinant human TFIIB protein fragments expressed in E.coli.
UniProt Number  Q00403
GeneBank ID  NM_001514
Subcellular location  Nucleus
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  TF2B; TFIIB
Gene_symbol  TF2B; TFIIB
Summary  GTF2B is one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.
UniPort summary  Function  General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Application Images :  
Western blot detection of TFIIB in Jurkat,MCF7&Hela cell lysates using TFIIB antibody (1:1000 diluted).
Predicted band size: 35KDa
Observed band size: 37KDa
Immunocytochemistry of HeLa cells using anti- TFIIB antibody diluted 1:50.
IHC of paraffin-embedded human colon using anti- TFIIB diluted 1/500-1/1000.