Size  100ug
Cat code  200808
Species Cross-reactivity  H,M
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2a
Molecular Weight  26kDa
Sensitivity  This antibody detects endogenous levels of LIN28A and does not cross-react with related proteins.
Immunogen  Purified recombinant human LIN28A protein fragments expressed in E.coli
UniProt Number  Q9H9Z2
GeneBank ID  NM_024674
Subcellular location  Cytoplasm.Nucleus
Formulation  Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide and 50% glycerol.
Altername  CSDD1;FLJ12457;LIN 28;Lin-28A;LIN28;LIN28A;LN28A_HUMAN;Protein lin-28 homolog A;ZCCHC1;Zinc finger CCHC domain-containing protein 1.
Gene_symbol  CSDD1;LIN28;LIN-28;ZCCHC1;lin-28A.
Summary  null
UniPort summary  Function  Acts as a ´translational enhancer´, driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis.Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and,indirectly,in stabilizing the mRNAs.Binds IGF2 mRNA,MYOD1 mRNA,ARBP/36B4 ribosomal protein mRNA and its own mRNA.Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression By similarity.Acts as a suppressor of microRNA(miRNA)biogenesis by specifically binding the precursor let-7(pre-let-7),a miRNA precursor.Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase,leading to the terminal uridylation of pre-let-7.Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation.Degradation of pre-let-7 in embryonic stem(ES)cells contributes to the maintenance of ES cells.In contrast,LIN28A down-regulation in neural stem cells by miR-125,allows the processing of pre-let-7.Specifically recognizes the 5´-GGAG-3´ motif in the terminal loop of pre-let-7.Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5´-GGAG-3´ motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
Application Images :  
Western blot detection of LIN28 in NTERA & F9 cell lysates using LIN28 antibody(1:1000 diluted).Predicted band size:26KDa,Observed band size:26KDa.
Western blot detection of LIN28A in LIN28B and LIN28A recombinant antigen fragments and using LIN28A antibody (1:1000 diluted).