Size  100ug
Cat code  200306
Species Cross-reactivity  H,Mk,R,M,Hm,G
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  37kDa
Sensitivity  This antibody detects endogenous levels GAPDH and does not cross-react with related proteins.
Immunogen  Purified recombinant human GAPDH protein fragments expressed in E.coli.
UniProt Number  P04406
GeneBank ID  NM_002046
Subcellular location  Cytoplasm >> cytosol.Nucleus.Cytoplasm >> perinuclear region.Membrane.Cytoplasm >> cytoskeleton.
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername  38 kDa BFA-dependent ADP-ribosylation substrate;Aging-associated gene 9 protein;BARS-38;cb609;EC 1.2.1.12;G3P_HUMAN;G3PD;G3PDH;GAPD ;GAPDH;GAPDH;Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle;Glyceraldehyde-3-phosphate dehydrogenase;KNC-NDS6;MGC102544;MGC102546;MGC103190;MGC103191;MGC105239;MGC127711 ;MGC88685;OCAS,p38 component;OCT1 coactivator in S phase,38-KD component;Peptidyl-cysteine S-nitrosylase GAPDH;wu:fb33a10.
Gene_symbol  G3PD;GAPD.
Summary  The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
UniPort summary  Function  Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Application Images :  
Western blot detection of GAPDH(Internal) antibody in SW480,A431,K562,Jurkat&Hela cell lysates using GAPDH(Internal) antibody (1:5000 diluted). 
Predicted band size: 37KDa 
Observed band size: 37KDa
Western blot detection of GAPDH(Internal) antibody in goat intestine,goat skeletal muscle,goat spleen & goat lung cell lysates using GAPDH(Internal) antibody (1:2000-1:5000 diluted).Predicted band size: 37KDa.Observed band size: 37KDa.
Western blot detection of GAPDH antibody in Jurkat,COS7,K562 and Hela cell lysates using GAPDH antibody (1:3000 diluted).Predicted band size:37KDa.Observed band size:37KDa.
Western blot detection of GAPDH antibody in 3T3,CHO-k1 and C6 cell lysates using GAPDH antibody (1:1000 diluted).Predicted band size:37KDa.Observed band size:37KDa.
Western blot detection of GAPDH antibody stability in K562 cell lysate(A：Stored at room temperature for 72 hours;B：Stored at -20℃) using GAPDH antibody (1:10000 diluted).Predicted band size:37KDa.Observed band size:37KDa.